Flp-intm t-rextm 293 细胞系
Web技术资料. 规格：. 1ml. Flp-In™系统使您可以稳定整合和表达感兴趣的基因，以提供单拷贝等基因细胞系。. Flp-In™表达会在您选择的哺乳动物细胞系基因组中引入一个Flp重组靶标（FRT）位点。. 随后，包含您感兴趣的基因的表达载体会通过FRT位点的Flp重组酶介 … WebFigure 5-9. PMA induces internalisation of hFFA4. Flp-InTM T-RExTM 293 cells able to express FLAG-hFFA4-mVenus were plated down onto poly-D-lysine-coated 30 mm glass coverslips and receptor expression was induced using doxycycline (100 ng/ml). The internalisation of hFFA4 in live cells was then imaged using a Zeiss VivaTome spinning …
Flp-intm t-rextm 293 细胞系
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WebFlp-In ™ 293 T-REx 细胞系设计用于快速生成稳定细胞系，确保通过 Flp-In™ 表达载体对您的目标蛋白质进行均一表达。这些细胞在转录活性基因组基因座处包含一个稳定整合的 … WebOct 31, 2024 · HEK293细胞及其衍生株HEK293（human embryonic kidney 293），简称293细胞，来源于人胚胎肾细胞，由于在19号染色体整合了大约4.35 kb的腺病毒基因组片段，使其细胞周期以及细胞表型、核型等发生了 ... 293FTM细胞是由Flp-InTM T-RExTM 293细胞导入单嗜性（ecotropic）报告质粒和 ...
WebFlp-In™ 293 T-REx cell lines is designed for rapid generation of stable cell lines that ensure homogenous expression of your protein of interest from a Flp-In™ expression … Flp-In™ 293 T-REx cell lines is designed for rapid generation of stable cell lines that … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes WebFlp-InTM T-RExTM 293 cells stably harboring hFFA2-eYFP at the Flp-InTM T-RExTM locus were induced to express the receptor. The capacity of GLPG0974 to inhibit elevation of [Ca2+] i. produced by EC80 concentrations of C3 or Cmp 1 is shown (B). Membranes from such induced cells were used to assess the ability of GLPG0974 to inhibit the binding of ...
WebMar 26, 2024 · Protocol 2 Protein complex purification from Flp-InTM T-RExTM 293 stably expressed FLAG tagged protein cell line Protocol 3 ChiP-Sequencing from Flp-InTM T-RExTM 293 stably expressed FLAG tagged protein cell lines Protocol 4 ChiP assay for endogenous gene Protocol 5 The cancer cell index, migration, and invasion assays Color … WebApr 2, 2024 · 基本信息. 商品名称：Epigenetics in Cancer 作者：编者:JUNJIANG FU//SABER IMANI 定价：198 出版社：科学 ISBN号：9787030543769 其他参考信息 （以实物为准）. 出版时间：2024-01-01 印刷时间：2024-01-01 版次：1 印次：1 开本：16开 包装：平装 页数：364 内容提要
Webgenomic location. To generate these cell lines, the Flp-In ™ T-REx System involves the following major steps: 1. Independent integration of the following two plasmids into the …
WebFeb 13, 2024 · flp/frt 系统就属于位点特异性重组系统的一种。 FLP是一个重组酶（flippase，Flp），类似于Cre，它识别的位点是FRT，当FRT在同一条DNA上且方向相 … sharon\u0027s schoonheidssalonWebT-REx™ 细胞系可稳定表达四环素抑制子蛋白（表 1）。使用 T-REx™ 系统可以节省大量的时间和精力。通过使用阳性对照载体 pcDNA™4⁄TO⁄lacZ 进行瞬时转染，对 T-REx™ 细胞系进行功能检测。在用四环素诱导时，T-REx™ 细胞系在抑制状态下表现出极低的基础表达水平，表达水平较高（图 1）。 sharon\\u0027s sewingWebThe Flp-In ™ T‑REx -293 cell line is shipped on dry ice. Store in liquid nitrogen vapor-phase upon receipt. The Flp-In™ T‑REx™-293 cell line is supplied as one vial containing 1 × … sharon\\u0027s shaved iceWebFeb 20, 2024 · 在我们以前的工作中，我们在四环素诱导型CMV启动子（HEK-293 Flp-In T-REx-Thermo）的控制下，建立了表达C末端3xFLAG标记的外来体成分EXOSC10（RRP6）的同基因HEK-293细胞系费雪科学）。. 该系统允许我们以与内源WT蛋白质相当的水平表达标记的EXOSC10蛋白质，并且使用磁性 ... sharon\\u0027s sewing camillusWebThe Flp-InTM T-RExTM 293 cells were generated by cotransfection of the constructed plasmid and pOG44 plasmid to express the target gene. Successfully recombined monoclonal cell lines were screened by hygromycin B resistance. Western blot and indirect immunofluorescence (IFA) were used to examine the expression of the target protein in ... sharon\u0027s scranton paWebAug 5, 2024 · 293Flp-InTMT-RExTM Cells. 293Flp-In 293 T-REx细胞系是为了快速生成稳定的细胞系而设计的，它可以确保从Flp-In表达载体上获得的蛋白的均匀表达。这些细胞在转录活跃的基因组位点上包含一个稳定整合的FRT位点，有针对性的整合Flp-In表达载体，确保高水平目的基因。 porche moderneWebFigure 4-2. TUG-891 but not TUG-1197 displays low potency mediated activation of hFFA1. Flp-InTM T-RExTM 293 cells able to express hFFA1-mVenus-HA were seeded (75,000 cells /well) into poly-D-lysine coated black clear-bottom 96-well plates. Expression of hFFA1 was induced by the antibiotic doxycycline (100 ng/ml) for 18-24 hours. Cells were … porch emser