Dynein intermediate chain binding
WebDynein axonemal heavy chain 5 is a protein that in humans is encoded by the DNAH5 gene. [5] [6] [7] DNAH5 is a protein-coding gene. 1 It provides the instructions for synthesizing a protein that belongs to a microtubule-associated protein complex made of heavy, light and intermediate chains. 2 DNAH5 is responsible for making the heavy … WebDec 8, 2024 · Dynein_heavy; Dynein heavy chain and region D6 of dynein motor pfam08393 Location: 763 → 1162 DHC_N2; Dynein heavy chain, N-terminal region 2 pfam12777 Location: 2586 → 2933 MT; Microtubule-binding stalk of dynein motor pfam12781 Location: 2962 → 3178 AAA_9; ATP-binding dynein motor region D5 …
Dynein intermediate chain binding
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WebWe show that human dynein light intermediate chain (DLIC) binds the N-terminal RH1 domain of JIP3, its paralog JIP4, and the lysosomal adaptor RILP. A point mutation in RH1 abrogates DLIC binding without perturbing the interaction between JIP3’s RH1 domain and kinesin heavy chain. WebMar 21, 2024 · Dynein will initiate translocation of late endosomes to microtubule minus ends only after interacting with betaIII spectrin, which requires the activities of Rab7 …
WebThe dynein light intermediate chain . 2. The dynein accessory proteins Lis1, Nde and Ndel-1 . This will be achieved by studying and manipulating dynein and accessory protein activity using in vitro motility assays and living tissue culture cells. Importantly, we will also investigate the role of WebAuthor(s): Rahman, Farhana; Johnson, Jennifer L; Zhang, Jinzhong; He, Jing; Pestonjamasp, Kersi; Cherqui, Stephanie; Catz, Sergio D Abstract: The dynein motor protein complex is required for retrograde transport but the functions of the intermediate-light chains that form the cargo-binding complex are not elucidated and the importance …
WebJul 13, 2001 · Previously, we identified dynactin as a cargo receptor or adaptor for cytoplasmic dynein, mediated by an interaction between the dynein intermediate chain … WebApr 7, 2006 · Cytoplasmic dynein is the multisubunit protein complex responsible for many microtubule-based intracellular movements. Its cargo binding domain consists of dimers of five subunits: the intermediate chains, the light intermediate chains, and the Tctex1, Roadblock, and LC8 light chains. The intermediate chains have a key role in the …
WebDynein Axonemal Light Chain 4 (DNAL4) protein is understood to be part of the axonemal (or ciliary and flagellar) complex of dynein molecules (including dynein heavy and light chains) (GO:0005858) and a component of the microtubule-based dynein motor complex [13].In humans, two known axonemal light chain proteins, DNAL1 and DNAL4, sit at the …
WebJul 13, 2001 · Insights into cytoplasmic dynein regulation have the potential to clarify the interactions necessary for cargo binding. In this study we explored the role of IC phosphorylation in regulating the IC-p150 Glued interaction. We mapped the site of IC phosphorylation, generated site-directed mutants, and tested the impact of these … on reed\\u0027sWebOct 1, 2014 · The LIC domain structure has interesting parallels with the dynein intermediate chain, which has a well-ordered WD repeat domain that interacts with the heavy chain and a less conserved disordered … inyectable celestoneWeb1 day ago · All light and intermediate chains are present, and only the ODA-DC complex is missing (Rao et al, 2024). ... Step 2: The β-HC, from helical bundle 5 to the β-dynein head, shifts slightly. The two binding partners LC3 and the α kelch domain move with the β-tail. Step 3: The stalk and MTBD of the α-HC shifts. ... inyectable hierroWebApr 26, 2024 · Cytoplasmic dynein belongs to the AAA+ superfamily of proteins. 13 There are nine subfamilies of dynein consisting of two cytoplasmic and seven axonemal dyneins. 12 A homodimer that is composed of two heavy chains (DHC, encoded by DYNC1H), two light intermediate chains (DLIC, encoded by DYNC1LI), four intermediate chains … on referent\u0027sWeb1 day ago · All light and intermediate chains are present, and only the ODA-DC complex is missing (Rao et al, 2024). ... Step 2: The β-HC, from helical bundle 5 to the β-dynein … on referent\\u0027sWebCytoplasmic dynein and dynactin are megadalton-sized multisubunit molecules that function together as a cytoskeletal motor. In the present study, we explore the mechanism of dynein-dynactin binding in vitro and then extend our findings to an in vivo context. Solution binding assays were used to define binding domains in the dynein intermediate chain (IC) and … inyectable epoWebMar 29, 2024 · Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the … inyectable intradermica